Lambda foo: a lambda phage vector for the expression of foreign proteins.
نویسندگان
چکیده
منابع مشابه
A programme for the construction of a lambda phage.
Infection of a sensitive host by the lambdoid coliphages can cause death of the bacterial cell by lysis or can lead to a lysogenic cell, with the viral DNA stably integrated into the host chromosome. These alternative responses both require the coordination of several host and phage functions, and lambda infection follows a well controlled developmental plan. The lytic and lysogenic pathways of...
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In the absence of RecA-mediated cleavage of the repressor, the lambda prophage is exceptionally stable. We develop a stochastic model that predicts the stability of such epigenetic states from affinities of the molecular components. We find that the stability, in particular, depends on the maximum possible cI protein production, and on the number of translated cro proteins per transcribed mRNA....
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Highly purified HindIII restriction fragments of Xenopus laevis 5S DNA and of Psammechinus miliaris histone DNA have been covalently inserted into a derivative of phage lambda. This phage, genetically constructed by Murray et al. (1), contains only a single target for HindIII in the cI gene. Viable hybrid molecules were detected as clear plaque-forming phage after transfection of E. coli, the v...
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The aim of the present work is to introduce the concept of $lambda _{r}$-almost convergence of sequences. We define the spaces $fleft( lambda _{r}right) $ and $f_{0}left( lambda _{r}right) $ of $ lambda _{r}$-almost convergent and $lambda _{r}$-almost null sequences. We investigate some inclusion relations concerning those spaces with examples and we determine the $beta $- and $gamma $-duals of...
متن کاملconstructing a dna ladder range for lambda phage by multiplex pcr
background and objectives: dna ladder contains dna fragments of different length but with known size, used to determine the size of unknown dna molecules. different dna ladders are available for expected dna length. conserved sequences were selected for design of primers to generate dna fragments of known specific size. materials and methods: in this study, we describe a method by which dna lad...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1994
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.91.17.8273